Statistics for Mask or Model objects
This dialog provides access to statistic values for shapes and intensities for model and mask objects.
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Contents
Parameters and Options

 10. A histogram plot that shows distribution of statistic parameters from 8.. The plot may be shown on a normal or logarithmic scale depending on selection of the Log scale check box (13.). Clicks with the left and right mouse allows to make selections.
 11. The Auto highlight on a click checkbox  when checked, each time the object in the table (8.) is selected it is highlighted in the Image View panel.
 12. The Log scale checkbox is for showing the histogram (8.) in the logarithmic scale.
 13. Two edit boxes are used to provides minimal and maximal values for highlighting after press of the Do button.
 14. The Run button starts quantification.
 15. The Details panel defines a way of object selection: the selected objects may be added, removed or used for replacement of the Selection layer.
 16. The Export button allows export of the statistic values to Excel or Matlab.
 17. The Sort button sorts the main table values; use a popup menu via the right mouse button to define a column to be sorted.
Statistical properties of 3D objects
Calculate properties of 3D objects of the dataset.
 Area  calculates total number of pixels (~volume) within the 3D objects.
 Endpoints Length  calculates the distance between two end points of a line segment. Please note that the width of the line should be 1. It is recommended to use Membrane ClickTracker tool in the Segmentation panel with the option Straight line and Width=1. Note! The resulting numbers are calculated in the image units.
 EquatorialEccentricity  returns the Equatorial Eccentricity, defined as the eccentricity of the section through the second longest and the shortest axes. The resulted values between 1 (line) and 0 (sphere)
 FilledArea  calculates total number of pixels (~volume) within the filled 3D objects.
 Holes Area  finds cavities within 3D objects and calculate their total number of pixels (~volume).
 MajorAxisLength  returns the length of the major axis in pixels
 MeridionalEccentricity  returns Meriodional Eccentricity, defined as the eccentricity of the section through the longest and the shortest axes
 SecondAxisLength  returns the length of the second major axis in pixels
 ThirdAxisLength  returns the length of the minor axis in pixels
Statistical properties of 2D objects
Calculate properties of 3D objects of the dataset.
 Area  calculates total number of pixels within the 2D objects.
 ConvexArea  calculates total number of pixels of the smallest convex polygon that can contain the object.
 CurveLengthInPixels  calculate the length of curve objects in pixels. It is possible to measure the length of both closed and nonclosed curves. Please note! It is required that the curves are thinned (Menu>Selection>Morphological operations) and the branch points are removed (The branch points may be found by selecting material and run branch point detection, Menu>Selection>Morphological operations).
 CurveLengthInUnits  calculates the length of curved objects in physical units of the dataset. It is possible to measure the length of both closed and nonclosed curves. Please note! It is required that the curves are thinned (Menu>Selection>Morphological operations) and the branch points are removed (The branch points may be found by selecting material and run branch point detection, Menu>Selection>Morphological operations).
 Eccentricity  calculates eccentricity of the ellipse that has the same secondmoments as the object. The eccentricity is the ratio of the distance between the foci of the ellipse and its major axis length. The value is between 0 and 1. (0 and 1 are degenerate cases; an ellipse whose eccentricityis 0 is actually a circle, while an ellipse whose eccentricity is 1 is a line segment.)
 Endpoints Length  calculates the distance between two end points of a line segment. Please note that the width of the line should be 1. It is recommended to use Membrane ClickTracker tool in the Segmentation panel with the option Straight line and Width=1. If the lines were drawn with the Brush tool they have to be thinned. The thinning may be achieved with the Brush tool size 1 or/and Thinning the objects using Menu>Selection>Morphological 2D operations>Thin. Note! The resulting numbers are calculated in the image units.
 EquivDiameter  calculates the diameter of a circle with the same area as the object. Computed as sqrt(4*Area/pi).
 EulerNumber  calculates the number of objects in the region minus the number of holes in those objects, i.e. the object with 1 hole has the Euler number 0, while the object with 2 holes has the Euler number 1.
 Extent  calculates the ratio of pixels in the object to pixels in the total bounding box. Computed as the Area divided by the area of the bounding box.
 FirstAxisLength  length of the major 2D axis of the object, in contrast to MajorAxisLength this property returns the real length of the object rather than that for the ellipse
 FilledArea  calculates the number of pixels of the filled object.
 Holes Area  finds holes and calculates total number of pixels within them.
 MajorAxisLength  calculates the length (in pixels) of the major axis of the ellipse that has the same normalized second central moments as the object.
 MinorAxisLength  calculate the length (in pixels) of the minor axis of the ellipse that has the same normalized second central moments as the object.
 Orientation  calculates the angle (in degrees ranging from 90 to +90 degrees) between the xaxis and the major axis of the ellipse that has the same secondmoments as the object.
 Perimeter  calculates the distance around the boundary of the object. It is computed as the perimeter by calculating the distance between each adjoining pair of pixels around the border of the region
 SecondAxisLength  length of the minor 2D axis of the object, in contrast to MinorAxisLength this property returns the real length of the object rather than that for the ellipse
 Solidity  calculates the proportion of the pixels in the convex hull that are also in the object. Computed as Area/ConvexArea.
Statistical properties of intensities of 2D/3D objects
These properties are calculated when the Intensity radio button in the Mode panel (3.) is selected.
 MinIntensity  detects minimal value of the image intensity within 2D or 3D objects.
 MaxIntensity  detects maximal value of the image intensity within 2D or 3D objects.
 MeanIntensity  calculates average intensity of the image within 2D or 3D objects.
 StdIntensity  calculates standard deviation intensity of the image within 2D or 3D objects.
 SumIntensity  calculates sum of the image intensities within 2D or 3D objects.
 Correlation  calculates correlation between image intensities of the two selected color channels. See more in the help of Matlab corr2 function.
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