How to extract the blob of gray image ?

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Dear All ,
I want to extract blob of this image then measure the maximum major length.
I try some method but is is not work.
here is my code:
clc
clear
X = imread('img.tif')
X = imadjust(X);
% Threshold image - manual threshold
BW = im2gray(X) > 0;
% Open mask with disk
radius = 3;
decomposition = 0;
se = strel('disk', radius, decomposition);
BW = imopen(BW, se);
% Close mask with disk
radius = 5;
decomposition = 0;
se = strel('disk', radius, decomposition);
BW = imclose(BW, se)*255;
% imshow(BW)
props = regionprops(BW,'MajorAxisLength','BoundingBox')
[max_length, idx] = max([props.MajorAxisLength]);
% Get the bounding box of the biggest blob
bbox = props(idx).BoundingBox;
% Plot the rectangle around the biggest blob
figure;
imshow(BW);
hold on;
rectangle('Position', bbox, 'EdgeColor', 'r', 'LineWidth', 2);
Thanks and Regards!
Han

Accepted Answer

Image Analyst
Image Analyst on 19 Feb 2023
I'd try some denoising first, like medfilt2 or imnlmfilt. Then I'd try adjusting the thresholds with my interactive thresholding app:
Then use bwferet. Don't use MaxAxisLength of regionprops since that gives you the max axis length of an ellipse fitted to the shape rather than the actual caliper distance like bwferet gives you.
  1 Comment
Jenifer NG
Jenifer NG on 23 Feb 2023
Thanks you for your advice
I use the ImageSementer APP then app imcomplement then i can get the expected output by using bwferet .

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