Automated Analysis of Platelet Aggregation in Microfluidics

Automatic thresholding of fluorescence microscopy images in microfluidics to determine platelet coverage and aggregate size distribution.

Two scripts for the analysis of platelet aggregation in microfluidic channels after whole blood perfusion. The first script, “Platelet_Coverage”, automatically corrects for channel misalignment, determines a threshold using the triangle method, creates a black and white image representing platelet coverage and finally determines the platelet coverage. The second script, “Clot_Properties”, uses the black and white image from the first script to determine the platelet aggregate size distribution.

Triange Thresholding is done using a script of dr. Panneton (MathWorks, #28047) which is based on the original work of Zack, Gregory W., William E. Rogers, and S. A. Latt. "Automatic measurement of sister chromatid exchange frequency." Journal of Histochemistry & Cytochemistry 25.7 (1977): 741-753.