Standard Method for Detection of Focused Areas in Microscope Images?
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OkinawaDolphin
on 18 Apr 2017
Commented: Image Analyst
on 19 Apr 2017
Is there a gold standard for finding focused areas in images acquired by optical microscopes? Is there a contrast measure frequently used?
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Image Analyst
on 18 Apr 2017
If the "in focus" regions have higher texture then you can find those regions with a texture filter like stdfilt() or convolve with a Laplacian kernel and then threshold to find the "high texture" regions.
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Image Analyst
on 19 Apr 2017
It was just FYI - that there is some justification for the approach I suggested because other apps are doing similar things. So, try stdfilt() for starters.
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