How do I play a multiframe TIFF file as a video in matlab?

I want to detect features that show up in different frames.

[Mark Sherstan]

Should not be a problem. Please post your file so we can verify. Most likely the tiff file will be a 4 dimensional matrix where the first 3 matrices are some color space and the 4th one relates to the frame or time. You will just have to loop through that final index.

[AM]

Hi Mark, I have attached a sample video that I would like to play in matlab. I tried using the code posted below by KSSV but that also only shows one frame at a time.

Thank you for all your help!

Answer 1

[Walter Roberson]

filename = '0024_MMStack_Pos0.ome-2.tif';
info = imfinfo(filename);
numframe = length(info);
for K = 1 : numframe
    rawframes(:,:,:,K) = imread(filename, K);
end
cookedframes = mat2gray(rawframes);
implay(cookedframes)

[AM]

Thank you Walter, this worked wonderfully!

[AM]

Is is possible to draw circles at points (180, 183) and (190 , 203) in the same video ('0024_MMStack_Pos0.ome-2.tif') and have them show up in the video when I play it?

[Walter Roberson]

You can use insertShape from the Computer Vision toolbox to draw the circles into the arrays.

Answer 2

[KSSV]

tifffiles = dir('*.tiff') ;
N = length(tifffiles) ;
for i = 1:N 
    tifffile = tifffiles(i).name ;
    imshow(tifffile) ;
    pause(0.1)
end

[AM]

Hi, thank you for your response but I when I try using that I get this message:

'Warning: Can only display one frame from this multiframe file'

Any suggestions about how to go around that problem?

Thanks