Image segmentation in pap-smear image

Hello,

Currently I am doing a small project on image processing of cervical cancer pap-smear image in GUI matlab. I have doubt on how can I segment the nucleus from the cytoplasm separately. I searched a lot but i am not satisfied with it. I dont know how can i do this as i am completely new to this field. please help me on how can I use the matlab code for segmentation of nucleus and cytoplasm.

Answer 1

[Keerthana Chiruvolu]

Hi,

Kindly use the Image Segment App to draw a free hand Region of Interest (ROI) around the nucleus and create a binary mask. For performing any statistical analysis on the image, you can use this mask.

If you want to crop the free-hand ROI from the image, refer the following links

https://www.mathworks.com/matlabcentral/answers/17951-to-calculate-mean-of-roi-drawn-using-freehand-tool#answer_24213

https://www.mathworks.com/matlabcentral/answers/420364-how-to-crop-required-roi-from-an-image#comment_615850

[vidhya v]

Thank you for your response mam.

But mam, I am developing a graphical user interface for image processing. I have already tried ImageSegmenter App, but how can I segment different pap-smear in GUI. Is there any formula through which I can segment the nucleus and cytoplasm in images?

[Keerthana Chiruvolu]

Hi,

You may also use the MATLAB functions to create a mask instead of using Image Segmenter App.

Kindly refer the sample code in the attachment.

[vidhya v]

I am using R2019b version of MATLAB

[Rik]

Comment posted as flag by NAVNISH GOEL:

how to use cropROI.mlx in MATLAB 2017a.

[vidhya v]

thank you for your response sir.