my serine recombinase s expressed on a plasmid. its substrate (dna sequence flanked by it two recombinase attachment site) is on the same plasmid.
So, enzyme is being produced gradually with fixed amount of substrate.
aas bxb1 activity is embedded in the flipping reaction pointing a link between bxb1 species and flipping reaction is with no effect.
How could I account for increasing amount of bxb1 concentration in the cell? i .e How do I link bxb1 production to its Michaelis–Menten kinetics represented by flipping reaction?