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error while loading .mat file in google colab

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I have a .mat file which i need to open/load in google colab. the file size is 590MB. I have uploaded file on google colab from my local drive. Then I have used different codes like following,
from scipy.io import loadmat
data=loadmat('Perfect_H_40000.mat')
another one is,
from os.path import dirname, join as pjoin
import scipy.io as sio
data_dir = pjoin(dirname(sio.file), 'matlab', 'tests', 'data')
mat_fname = pjoin(data_dir, 'Perfect_H_40000.mat')
mat_contents = sio.loadmat(mat_fname)
there is another,
import h5py
f = h5py.File('Perfect_H_40000.mat')
but all the time I failed to load this .mat file. please suggest some suitable method. Thankyou

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